WDR41 Mouse Monoclonal Antibody [A9C10]
Catalog# HA601116
WDR41 Mouse Monoclonal Antibody [A9C10]
-
WB
-
IF-Cell
-
Human
-
unconjugated
Overview
Product Name
WDR41 Mouse Monoclonal Antibody [A9C10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human aa 231-430 / 459.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
Daudi cell lysate, SiHa cell lysate, Hela.
Conjugation
unconjugated
Clone Number
A9C10
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:200
Target
Function
Non-catalytic component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy 1, 2, 3, 4. The C9orf72-SMCR8 complex promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation 5. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity.
Background References
1. Tang D et al. The C9orf72-SMCR8-WDR41 complex is a GAP for small GTPases. Autophagy. 2020 Aug
2. Tang D et al. Cryo-EM structure of C9ORF72-SMCR8-WDR41 reveals the role as a GAP for Rab8a and Rab11a. Proc Natl Acad Sci U S A. 2020 May
Subcellular Location
Cytoplasm.
Synonyms
FLJ10904 antibody
MSTP048 antibody
WD repeat domain 41 antibody
WD repeat protein 41 antibody
WD repeat-containing protein 41 antibody
WDR 41 antibody
WDR41 antibody
WDR41_HUMAN antibody
Images
-
Western blot analysis of WDR41 on HeLa lysate with Mouse anti-WDR41 antibody (HA601116) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 3 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601116) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling WDR41 with Mouse anti-WDR41 antibody (HA601116) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WDR41 antibody (HA601116) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"