FIP200 Mouse Monoclonal Antibody [A9A10]
Catalog# HA601107
FIP200 Mouse Monoclonal Antibody [A9A10]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
FIP200 Mouse Monoclonal Antibody [A9A10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human RB1CC1 aa 651-800 / 1,594.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 183 kDa
Positive Control
Hela cell lysate, MG-63 cell lysate, MCF-7 cell lysate, 293T cell lysate, K562 cell lysate, A431 cell lysate, Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate.
Conjugation
unconjugated
Clone Number
A9A10
RRID
Product Features
Form
Liquid
Concentration
1.17 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
FIP200 (FAK family kinase-interacting protein of 200 kDa) was identified in a two-hybrid screen with the tyrosine kinase Pyk2 and can inhibit Pyk2 kinase activity as well as related family members. FIP200 was later independently identified in a multi-drug resistance screen and named RB1CC1 (RB1-inducible coiled-coil 1) due to its induction by cytotoxic stress and RB1 expression regulation. FIP200 function has been linked to apoptosis, cell cycle progression, cell growth, and migration. FIP200 has also recently been shown to interact with ULK1 and is required for autophagosome formation. FIP200 is part of an ULK1 complex along with Atg13 that is regulated by mTOR and is required for starvation induced autophagy.
Background References
1. Yeo SK et al. Role of FIP200 in inflammatory processes beyond its canonical autophagy function. Biochem Soc Trans. 2020 Aug
2. Wang L et al. FIP200 restricts RNA virus infection by facilitating RIG-I activation. Commun Biol. 2021 Jul
Subcellular Location
Nucleus, Cytoplasm, Cytoplasm, cytosol, Lysosome.
Synonyms
FAK family kinase-interacting protein of 200 kDa antibody
FIP200 antibody
RB1-inducible coiled-coil protein 1 antibody
RB1CC1 antibody
RBCC1_HUMAN antibody
Images
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Western blot analysis of FIP200 on different lysates with Mouse anti-FIP200 antibody (HA601107) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: MG-63 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: 293T cell lysate
Lane 5: K562 cell lysate
Lane 6: A431 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: HepG2 cell lysate
Lane 9: NIH/3T3 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 183 kDa
Observed band size: 250/150 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601107) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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