Cytokeratin 20 Recombinant Mouse Monoclonal Antibody [A3H7-R]
Catalog# HA601077
Cytokeratin 20 Recombinant Mouse Monoclonal Antibody [A3H7-R]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
Cytokeratin 20 Recombinant Mouse Monoclonal Antibody [A3H7-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within C-terminal Human Cytokeratin 20.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
Human colon tissue, human small intestine tissue.
Conjugation
unconjugated
Clone Number
A3H7-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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IF-Tissue
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1:500
Target
Function
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to CK20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the CK20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumour.
Background References
1. You X et al. Aberrant Cytokeratin 20 mRNA Expression in Peripheral Blood and Lymph Nodes Indicates Micrometastasis and Poor Prognosis in Patients With Gastric Carcinoma. Technol Cancer Res Treat. 2019 Jan.
2. Al-Maghrabi J et al. Immunohistochemical staining of cytokeratin 20 and cytokeratin 7 in colorectal carcinomas: Four different immunostaining profiles. Saudi J Gastroenterol. 2018 Mar-Apr.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
CK 20 antibody
CK-20 antibody
CK20 antibody
Cytokeratin-20 antibody
Cytokeratin20 antibody
K1C20_HUMAN antibody
K20 antibody
KA20 antibody
Keratin 20 antibody
keratin 20, type I antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Cytokeratin 20 antibody (HA601077) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-Cytokeratin 20 antibody (HA601077) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: small intestine
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Appendix
Sample: Paraffin-embedded section
Primary antibody dilution: 1/500
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes
Platform: Leica Biosystems BOND® RX
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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