TCP1 delta Mouse Monoclonal Antibody [A8E2]
Catalog# HA601064
TCP1 delta Mouse Monoclonal Antibody [A8E2]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TCP1 delta Mouse Monoclonal Antibody [A8E2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human TCP1 delta aa 200-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 58 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, PC-12 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, PC-3M cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, human breast carcinoma tissue, human stomach tissue.
Conjugation
unconjugated
Clone Number
A8E2
RRID
Product Features
Form
Liquid
Concentration
1.1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:800
Target
Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable). The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin, as well as alpha-transducin (MIM 139330).
Background References
1. Freund A., Zhong F.L., Venteicher A.S., Meng Z., Veenstra T.D., Frydman J., Artandi S.E. Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1. Cell 159:1389-1403(2014)
2. Seo S., Baye L.M., Schulz N.P., Beck J.S., Zhang Q., Slusarski D.C., Sheffield V.C. BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. Proc. Natl. Acad. Sci. U.S.A. 107:1488-1493(2010)
Subcellular Location
Cytoskeleton
Synonyms
CCT delta antibody
CCT4 antibody
CCTD antibody
chaperonin containing t-complex polypeptide 1, delta subunit antibody
chaperonin containing TCP1, subunit 4 (delta) antibody
SRB antibody
Stimulator of TAR RNA binding antibody
T complex protein 1 subunit delta antibody
TCP-1-delta antibody
Images
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Western blot analysis of TCP1 delta on different lysates with Mouse anti-TCP1 delta antibody (HA601064) at 1/1,000 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: A549 cell lysate (10 µg/Lane)
Lane 3: PC-12 cell lysate (10 µg/Lane)
Lane 4: SH-SY5Y cell lysate (10 µg/Lane)
Lane 5: THP-1 cell lysate (10 µg/Lane)
Lane 6: PC-3M cell lysate (10 µg/Lane)
Lane 7: Jurkat cell lysate (10 µg/Lane)
Lane 8: Mouse testis tissue lysate (20 µg/Lane)
Lane 9: Rat spleen tissue lysate (20 µg/Lane)
Lane 10: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 58 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601064) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-TCP1 delta antibody (HA601064) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601064) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-TCP1 delta antibody (HA601064) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601064) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"