IL-28A Mouse Monoclonal Antibody [A5D6]
Catalog# HA601027
IL-28A Mouse Monoclonal Antibody [A5D6]
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WB
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Human
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unconjugated
Overview
Product Name
IL-28A Mouse Monoclonal Antibody [A5D6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human IL28A aa 110-160.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 22 kDa
Positive Control
MCF7 cell lysate, HEK-293 cell lysate, SK-Br-3 cell lysate.
Conjugation
unconjugated
Clone Number
A5D6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500-1:1,000
Target
Function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
Background References
1. Sheppard P. et. al. IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat. Immunol. 4:63-68(2003).
Subcellular Location
Secreted
UNIPROT
Synonyms
Cytokine ZCYTO20 antibody
IFN-lambda-2 antibody
IFNL2 antibody
IL-28A antibody
IL28A antibody
IL28A_HUMAN antibody
Interferon lambda 2 antibody
Interferon lambda-2 antibody
interleukin 28A (interferon, lambda 2) antibody
Interleukin 28A antibody
ExpandImages
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Western blot analysis of IL-28A on different lysates with Mouse anti-IL-28A antibody (HA601027) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: SK-Br-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"