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CD42a Mouse Monoclonal Antibody [A5E6]

Usd: 350

Specification

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Catalog# HA601019

CD42a Mouse Monoclonal Antibody [A5E6]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CD42a Mouse Monoclonal Antibody [A5E6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human CD42a aa 1-150 / 177.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 19 kDa

Positive Control

MCF7 cell lysate, human lung tissue lysate, mouse spleen tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

A5E6

RRID

AB_3071743

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:2,000

Target

Function

Glycoprotein IX (platelet) (GP9) also known as CD42a (Cluster of Differentiation 42a), is a human gene. Platelet glycoprotein IX (GP9) is a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor(known as the Glycoprotein Ib-IX-V Receptor Complex). The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain and a beta chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein.

Background References

1. Martins Castanheira N et al. Uptake of platelets by cancer cells and recycling of the platelet protein CD42a. J Thromb Haemost. 2022 Jan

Subcellular Location

Membrane.

UNIPROT

SwissProt: P14770 Human

SwissProt: O88186 Mouse

Synonyms

CD42a antibody

Glycoprotein 9 antibody

Glycoprotein IX antibody

Glycoprotein IX (platelet) antibody

Glycoprotein IX precursor antibody

GP IX antibody

GP-IX antibody

GP9 antibody

GPIX antibody

GPIX_HUMAN antibody

Expand

Images

  • Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (<a href="/products/HA601019" style="font-weight: bold;text-decoration: underline;">HA601019</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: Human lung tissue lysate<br />Lane 3: Mouse spleen tissue lysate<br />Lane 4: Mouse lung tissue lysate<br />Lane 5: Rat lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 19 kDa<br />Observed band size: 28 kDa<br /><br />Exposure time: 1 minute 2 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601019" style="font-weight: bold;text-decoration: underline;">HA601019</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA601019) at 1/2,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: Human lung tissue lysate
    Lane 3: Mouse spleen tissue lysate
    Lane 4: Mouse lung tissue lysate
    Lane 5: Rat lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 19 kDa
    Observed band size: 28 kDa

    Exposure time: 1 minute 2 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601019) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (<a href="/products/HA601019" style="font-weight: bold;text-decoration: underline;">HA601019</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601019" style="font-weight: bold;text-decoration: underline;">HA601019</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA601019) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601019) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (<a href="/products/HA601019" style="font-weight: bold;text-decoration: underline;">HA601019</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601019" style="font-weight: bold;text-decoration: underline;">HA601019</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA601019) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601019) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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