IL-1 beta Mouse Monoclonal Antibody [A7F9] (HA601002)
Catalog# HA601002
Mouse Monoclonal to IL-1 beta
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WB
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ELISA
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IF-Cell
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Human
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Mouse
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Rat
Western blot analysis of IL-1 beta on different lysates with Mouse anti-IL-1 beta antibody (HA601002) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31/28/20 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601002) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
IL-1 beta Mouse Monoclonal Antibody [A7F9] (HA601002)
Immunogen
Recombinant protein within human il-1 beta aa 117-269 / 269.
Host
Mouse
Positive Control
THP-1 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate, RAW264.7 treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours cell lysate, HeLa cells treated with 80nM TPA overnight then replaced with 100ng/mL LPS for 6 hours add 300ng/mL BFA for last 3 hours.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A7F9
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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ELISA
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1:20,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
IL-1 beta
SYNONYMS
Catabolin antibody;H1 antibody;IFN beta inducing factor antibody;IL 1 antibody;IL 1 beta antibody;IL-1 beta antibody;IL1 antibody;IL1 BETA antibody;IL1B antibody;IL1B_HUMAN antibody;IL1F2 antibody;Interleukin 1 beta antibody;Interleukin 1 beta precursor antibody;interleukin 1, beta antibody;Interleukin-1 beta antibody;OAF antibody;Osteoclast activating factor antibody;OTTHUMP00000162031 antibody;Preinterleukin 1 beta antibody;Preinterleukin beta antibody;Pro interleukin 1 beta antibody
SUBCELLULAR LOCATION
Extracellular exosome, Secreted, Lysosome, Cytosol.
FUNCTION
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
CITATIONS
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Complement C5 activation promotes type 2 diabetic kidney disease via activating STAT3 pathway and disrupting the gut‐kidney axis
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IHC
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Mouse
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Citation
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Microglial responses and pain behaviors are exacerbated by chronic sleep deprivation in rats with chronic pain via neuroinflammatory pathways
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WB
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Rat
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Citation
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Inhibition of P2X7 Purinergic Receptor Ameliorates Cardiac Fibrosis by Suppressing NLRP3/IL-1β Pathway
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WB
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Mouse
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Citation
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