PDE4D Mouse Monoclonal Antibody [A7F3]
Catalog# HA601001
PDE4D Mouse Monoclonal Antibody [A7F3]
-
WB
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
PDE4D Mouse Monoclonal Antibody [A7F3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human PDE4D aa 610-809 / 809.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 91 kDa
Positive Control
HeLa cell lysate, HT-29 cell lysate, C2C12 cell lysate.
Conjugation
unconjugated
Clone Number
A7F3
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. cAMP-specific 3',5'-cyclic phosphodiesterase 4D is an enzyme that in humans is encoded by the PDE4D gene. The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells. PDE4D has been shown to interact with myomegalin and GNB2L1. Mutations in this gene have been associated to cases of acrodysostosis. This is the subtype of PDE4 that appears to be involved in the emetic and antidepressant effects of PDE4 inhibitors. Furthermore, changes in expression of the isoform PDE4D7 have been proposed as prostate cancer biomarker.
Background References
1. Wang Y. et. al. A Novel PDE4D Inhibitor BPN14770 Reverses Scopolamine-Induced Cognitive Deficits via cAMP/SIRT1/Akt/Bcl-2 Pathway. Front Cell Dev Biol. 2020 Dec
2. Leslie SN. et. al. Phosphodiesterase PDE4D Is Decreased in Frontal Cortex of Aged Rats and Positively Correlated With Working Memory Performance and Inversely Correlated With PKA Phosphorylation of Tau. Front Aging Neurosci. 2020 Oct
Subcellular Location
Apical cell membrane, Cytoplasm, Cytoskeleton, Centrosome, Membrane.
Synonyms
cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
DKFZp686M11213 antibody
5''-cyclic phosphodiesterase 4D antibody
cAMP specific phosphodiesterase 4D antibody
cAMP specific phosphodiesterase PDE4D6 antibody
cAMP-specific 3'' antibody
DPDE3 antibody
Dunce like phosphodiesterase E3 antibody
FLJ97311 antibody
HSPDE4D antibody
ExpandImages
-
Western blot analysis of PDE4D on different lysates with Mouse anti-PDE4D antibody (HA601001) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HT-29 cell lysate
Lane 3: C2C12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 85/91 kDa
Exposure time: Lane 1: 1 minute 2 seconds; Lane 2-3: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601001) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
