iFluor™ 555 Conjugated pan Cytokeratin Recombinant Mouse Monoclonal Antibody [PDH09-10] (HA600134F)
Catalog# HA600134F
Mouse Monoclonal to pan Cytokeratin
Tested for
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 555
Immunocytochemistry analysis of A431 cells labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600134F) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Cytokeratin antibody (HA600134F, iFluor™ 555) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Cytokeratin antibody (HA600134F, iFluor™ 555) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.