iFluor™ 594 Conjugated beta Tubulin Recombinant Mouse Monoclonal Antibody [A1-A4-R] (HA600110F)
Catalog# HA600110F
Mouse Monoclonal to beta Tubulin
Tested for
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IF-Cell
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IF-Tissue
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FC
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 594
Immunocytochemistry analysis of HeLa cells labeling Beta tubulin with Mouse anti-Beta tubulin antibody (HA600110F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Beta tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.