BSA Mouse Monoclonal Antibody [A7D11]
Catalog# HA600086
BSA Mouse Monoclonal Antibody [A7D11]
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ELISA
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WB
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Cow
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unconjugated
Overview
Product Name
BSA Mouse Monoclonal Antibody [A7D11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Native protein.
Species Reactivity
Cow
Validated Applications
ELISA, WB
Molecular Weight
Predicted band size: 66 kDa
Positive Control
BSA protein.
Conjugation
unconjugated
Clone Number
A7D11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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ELISA
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1:10,000
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WB
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1:500
Target
Function
Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2+, Na+, K+, fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds. BSA Antibody is a high quality monoclonal BSA antibody (also designated Fraction V antibody, ALB antibody, or Bos Taurus Serum Albumin antibody) suitable for the detection of the BSA protein of bovine origin. Anti-bovine serum albumin (anti-BSA) antibody can be used for the detection of BSA in tracer studies or in protease assays. This antibody demonstrates high specificity for BSA and no crossreactivity to HSA (Human serum albumin), Goat serum and Rabbit serum.
Background References
1. Ambrósio JAR. et. al. BSA nanoparticles loaded-methylene blue for photodynamic antimicrobial chemotherapy (PACT): effect on both growth and biofilm formation by Candida albicans. J Biomater Sci Polym Ed. 2020 Dec
Subcellular Location
Secreted.
UNIPROT
Synonyms
Albumin
BSA
Bos d 6
ALB
Images
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Bovine Serum Albumin Antibody (HA600086) in indirect ELISA.
Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Bovine Serum Albumin Antibody (HA600086) in indirect ELISA.
Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 0.8 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
This antibody demonstrates high specificity for BSA and little or no crossreactivity to HSA (Human serum albumin), Goat serum and Rabbit serum. -
Western blot analysis of BSA on BSA protein with Mouse anti-BSA antibody (HA600086) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600086) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"