Glucosidase 2 subunit beta Mouse Monoclonal Antibody [A6H9]

Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA600054) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Daudi cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A431 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 80 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA600054) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 70 kDa

Exposure time: 9 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600054) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Hela cells labeling Glucosidase 2 subunit beta with Mouse anti-Glucosidase 2 subunit beta antibody (HA600054) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Glucosidase 2 subunit beta antibody (HA600054) at 1/100 dilution in 1% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-Glucosidase 2 subunit beta antibody (HA600054) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA600054) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of Hela cells labeling Glucosidase 2 subunit beta.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA600054, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).