PCBP1 Mouse Monoclonal Antibody [A3E10]
Catalog# HA600019
PCBP1 Mouse Monoclonal Antibody [A3E10]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
PCBP1 Mouse Monoclonal Antibody [A3E10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human PCBP1 aa 1-356.
Species Reactivity
Human (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
37 kDa
Positive Control
Hela cell lysate, K562 cell lysate, Human colon cancer tissue.
Conjugation
unconjugated
Clone Number
A3E10
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:100-1:500
Target
Function
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP2 and HNRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.
Background References
1. Walter B.L. et. al. Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication. J. Virol. 76:12008-12022(2002).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
Alpha-CP1 antibody
Heterogeneous nuclear ribonucleoprotein E1 antibody
heterogenous nuclear ribonucleoprotein E1 antibody
heterogenous nuclear ribonucleoprotein X antibody
hnRNP E1 antibody
hnRNP-E1 antibody
hnRNP-X antibody
HNRPE1 antibody
HNRPX antibody
nucleic acid binding protein sub 2.3 antibody
ExpandImages
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Western blot analysis of PCBP1 on different lysates with Mouse anti-PCBP1 antibody (HA600019) at 1/1,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600019, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37.5 kDa
Observed band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using anti-PCBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600019, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"