Hamartin Mouse Monoclonal Antibody [A6F1]
Catalog# HA600004
Hamartin Mouse Monoclonal Antibody [A6F1]
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WB
-
IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Hamartin Mouse Monoclonal Antibody [A6F1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human Hamartin aa 401-600.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 130 kDa
Positive Control
293T (Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate,Mouse brain tissue lysate, human kidney tissue, human fetal skeletal muscle tissue.
Conjugation
unconjugated
Clone Number
A6F1
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500-1:5000
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IHC-P
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1:200-1:600
Target
Function
Tuberous sclerosis 1 (TSC1), also known as hamartin, is a protein that in humans is encoded by the TSC1 gene. TSC1 functions as a co-chaperone which inhibits the ATPase activity of the chaperone Hsp90 (heat shock protein-90) and decelerates its chaperone cycle. Tsc1 functions as a facilitator of Hsp90 in chaperoning the kinase and non-kinase clients including Tsc2, therefore preventing their ubiquitination and degradation in the proteasome. TSC1, TSC2 and TBC1D7 is a multi-protein complex also known as the TSC complex. This complex negatively regulates mTORC1 signaling by functioning as a GTPase-activating protein (GAP) for the small GTPase Rheb, an essential activator of mTORC1. The TSC complex has been implicated as a tumor suppressor. Defects in this gene can cause tuberous sclerosis, due to a functional impairment of the TSC complex. Defects in TSC1 may also be a cause of focal cortical dysplasia. TSC1 may be involved in protecting brain neurons in the CA3 region of the hippocampus from the effects of stroke.
Background References
1. Volovelsky O. et. al. Hamartin regulates cessation of mouse nephrogenesis independently of Mtor. Proc Natl Acad Sci U S A. 2018 Jun
2. Jozwiak J. Hamartin and tuberin: working together for tumour suppression. Int J Cancer. 2006 Jan
Subcellular Location
Cytoplasm, Membrane.
Synonyms
Hamartin antibody
kiaa0243 antibody
LAM antibody
TSC antibody
Tsc1 antibody
Tsc1 gene antibody
TSC1_HUMAN antibody
Tuberous sclerosis 1 antibody
Tuberous sclerosis 1 protein antibody
tumor suppressor antibody
Images
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Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (HA600004) at 1/5,000 dilution.
Lane 1: 293T (Human embryonic kidney cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600004, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 130 kDa
Observed band size: 150 kDa -
Western blot analysis of Hamartin on Mouse brain tissue lysate with Rabbit anti-Hamartin antibody (HA600004) at 1/5,000 dilution.
Lysates/proteins at 30 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600004, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 130 kDa
Observed band size: 150 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Hamartin antibody (HA600004) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600004) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue with Mouse anti-Hamartin antibody (HA600004) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Hamartin Recombinant Rabbit Monoclonal Antibody [PSH14-67]
Application: WB,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
Products with the same target and pathway
Hamartin Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Hamartin Recombinant Rabbit Monoclonal Antibody [PSH14-67]
Application: WB,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
