PELO Rabbit Polyclonal Antibody (HA500548)
Catalog# HA500548
Rabbit Polyclonal to PELO
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of PELO on different lysates with Rabbit anti-PELO antibody (HA500548) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500548) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500548) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.