PELO Rabbit Polyclonal Antibody
Catalog# HA500548
PELO Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
PELO Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PELO aa 1-385.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C6 cell lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
0.4 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Protein pelota homolog is a protein that in humans is encoded by the PELO gene. This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. In yeasts, the Dom34-Hbs1 complex (with ABCE1) that it forms is responsible for reactivating ribosomes and for recovering those stuck on mRNAs. It is a paralog of the release factor eRF1.
Background References
1. Wu X et al. Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity. Immunity. 2023 May
2. Hao J et al. PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10. FEBS J. 2024 Aug
Subcellular Location
Cytoplasm.
Synonyms
CGI 17 antibody
CGI17 antibody
pelo antibody
PELO_HUMAN antibody
pelota (Drosophila) homolog antibody
pelota homolog antibody
PRO1770 antibody
Protein pelota homolog antibody
Images
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Western blot analysis of PELO on different lysates with Rabbit anti-PELO antibody (HA500548) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500548) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"