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PORCN Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500546

PORCN Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PORCN Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 52 kDa

Positive Control

K-562 cell lysate, HepG2 cell lysate, SiHa cell lysate, 293T cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Peptide affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

PORCN (porcupine homolog – Drosophila) is a human gene. The protein is homologous to other membrane-bound O-acyltransferases. The protein encoded by this gene is an endoplasmic reticulum transmembrane protein involved in processing of wingless proteins such as WNT7A. It performs O-Palmitoleoylation of these proteins.

Background References

1. Wu S et al. PORCN promotes hepatocellular carcinogenesis via Wnt/beta-catenin-dependent manner. Transl Cancer Res. 2023 Jul

2. Fuhrmann S et al. Porcn is essential for growth and invagination of the mammalian optic cup. Front Cell Dev Biol. 2022 Oct

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q9H237 Human

SwissProt: Q9JJJ7 Mouse

Entrez Gene: 317368 Rat

Synonyms

DHOF antibody

FODH antibody

MG61 antibody

MGC29687 antibody

por antibody

PORC antibody

PORCN antibody

PORCN_HUMAN antibody

PPN antibody

Probable protein-cysteine N-palmitoyltransferase porcupine antibody

Expand

Images

  • Western blot analysis of PORCN on different lysates with Rabbit anti-PORCN antibody (<a href="/products/HA500546" style="font-weight: bold;text-decoration: underline;">HA500546</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: SiHa cell lysate (20 µg/Lane)<br />Lane 4: 293T cell lysate (20 µg/Lane)<br />Lane 5: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 6: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 7: C6 cell lysate (20 µg/Lane)<br />Lane 8: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 9: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 10: Rat brain tissue lysate (40 µg/Lane)<br />Lane 11: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 52 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500546" style="font-weight: bold;text-decoration: underline;">HA500546</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PORCN on different lysates with Rabbit anti-PORCN antibody (HA500546) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: SiHa cell lysate (20 µg/Lane)
    Lane 4: 293T cell lysate (20 µg/Lane)
    Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 6: Neuro-2a cell lysate (20 µg/Lane)
    Lane 7: C6 cell lysate (20 µg/Lane)
    Lane 8: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 9: Mouse brain tissue lysate (40 µg/Lane)
    Lane 10: Rat brain tissue lysate (40 µg/Lane)
    Lane 11: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 52 kDa
    Observed band size: 37 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500546) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"