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Orosomucoid 2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500537

Orosomucoid 2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Orosomucoid 2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Orosomucoid 2 aa 20-201 / 201.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 24 kDa

Positive Control

HepG2 cell lysate, LO2 cell lysate, Huh7 cell lysate, A549 cell lysate, HepG2, Huh7.

Conjugation

unconjugated

RRID

AB_3096659

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:250

  • FC

  • 1:1,000

Target

Function

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

Background References

1. Zhou B et al. Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis. Nat Metab. 2022 Sep

2. Li L et al. Mitigation of non-alcoholic steatohepatitis via recombinant Orosomucoid 2, an acute phase protein modulating the Erk1/2-PPARγ-Cd36 pathway. Cell Rep. 2023 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P19652 Human

Synonyms

AGP B' antibody

AGP 2 antibody

AGP B antibody

AGP2 antibody

Alpha 1 acid glycoprotein 2 antibody

Alpha 1 acid glycoprotein type 2 antibody

OMD 2 antibody

ORM 2 antibody

Orosomucoid2 antibody

Images

  • Western blot analysis of Orosomucoid 2 on different lysates with Rabbit anti-Orosomucoid 2 antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: LO2 cell lysate<br />Lane 3: Huh7 cell lysate<br />Lane 4: A549 cell lysate<br /><br />Lysates/proteins at 25 µg/Lane.<br /><br />Predicted band size: 24 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 25 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Orosomucoid 2 on different lysates with Rabbit anti-Orosomucoid 2 antibody (HA500537) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: LO2 cell lysate
    Lane 3: Huh7 cell lysate
    Lane 4: A549 cell lysate

    Lysates/proteins at 25 µg/Lane.

    Predicted band size: 24 kDa
    Observed band size: 54 kDa

    Exposure time: 25 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500537) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HepG2 cells labeling Orosomucoid 2 with Rabbit anti-Orosomucoid 2 antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Orosomucoid 2 antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HepG2 cells labeling Orosomucoid 2 with Rabbit anti-Orosomucoid 2 antibody (HA500537) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Orosomucoid 2 antibody (HA500537) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of Huh7 cells labeling Orosomucoid 2 with Rabbit anti-Orosomucoid 2 antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Orosomucoid 2 antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Huh7 cells labeling Orosomucoid 2 with Rabbit anti-Orosomucoid 2 antibody (HA500537) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Orosomucoid 2 antibody (HA500537) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HepG2 cells labeling Orosomucoid 2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling Orosomucoid 2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500537, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of Huh7 cells labeling Orosomucoid 2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500537" style="font-weight: bold;text-decoration: underline;">HA500537</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Huh7 cells labeling Orosomucoid 2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500537, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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