Nucleoporin 107 (Nup107) is a protein that in humans is encoded by the NUP107 gene. This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Background References
1. Shore T et al. Nucleoporin107 mediates female sexual differentiation via Dsx. Elife. 2022 Mar
2. Shi R et al. Nucleoporin 107 Promotes the Survival of Tumor Cells in Cervical Cancers. Gynecol Obstet Invest. 2020
Western blot analysis of Nup107 on different lysates with Rabbit anti-Nup107 antibody (HA500521) at 1/1,000 dilution.
Lane 1: HeLa cell lysate(20 µg/Lane) Lane 2: HepG2 cell lysate(20 µg/Lane) Lane 3: RAW264.7 cell lysate(20 µg/Lane) Lane 4: NIH/3T3 cell lysate(20 µg/Lane) Lane 5: PC-12 cell lysate(20 µg/Lane) Lane 6: mouse testis tissue lysate(30 µg/Lane) Lane 7: rat testis tissue lysate(30 µg/Lane)
Exposure time: 4 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500521, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 106 kDa Observed band size: 106 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"