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Gbeta5 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500513

Gbeta5 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Gbeta5 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Gbeta5 aa 1-200.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 44 kDa

Positive Control

HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat brain tissue, Hela.

Conjugation

unconjugated

RRID

AB_3071598

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

Target

Function

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist.

Background References

1. Mai JH. et. al. Intellectual developmental disorder with cardiac arrhythmia syndrome in a family caused by GNB5 variation and literature review. Zhonghua Er Ke Za Zhi. 2020 Oct

2. Shao Z. et. al. Unique retinal signaling defect in GNB5-related disease. Doc Ophthalmol. 2020 Jun

Subcellular Location

Membrane.

UNIPROT

SwissProt: O14775 Human

SwissProt: P62881 Mouse

SwissProt: P62882 Rat

Synonyms

FLJ37457 antibody

FLJ43714 antibody

G protein beta 5 subunit antibody

G protein beta subunit 5L antibody

GB 5 antibody

GB5 antibody

GBB5_HUMAN antibody

Gbeta5 antibody

GNB 5 antibody

GNB5 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Gbeta5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500513" style="font-weight: bold;text-decoration: underline;">HA500513</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Gbeta5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500513, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Gbeta5 on different lysates with Rabbit anti-Gbeta5 antibody (<a href="/products/HA500513" style="font-weight: bold;text-decoration: underline;">HA500513</a>) at 1/5,000 dilution.<br /><br />Lane 1: mouse brain tissue lysate <br />Lane 2: rat brain tissue lysate<br /><br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500513" style="font-weight: bold;text-decoration: underline;">HA500513</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 44 kDa<br />Observed band size: 40 kDa

    Western blot analysis of Gbeta5 on different lysates with Rabbit anti-Gbeta5 antibody (HA500513) at 1/5,000 dilution.

    Lane 1: mouse brain tissue lysate
    Lane 2: rat brain tissue lysate


    Lysates/proteins at 20 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500513, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 44 kDa
    Observed band size: 40 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"