CNTFR Rabbit Polyclonal Antibody
Catalog# HA500506
CNTFR Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CNTFR Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human CNTFR aa 201-250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, rat brain tissue lysate,rat cerebellum tissue lysate, human fetal skeletal muscle tissue, mouse heart tissue, Hela, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:200
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IHC-P
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1:200
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FC
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1:500-1:1,000
Target
Function
This gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of a tripartite receptor for ciliary neurotrophic factor, which plays a critical role in neuronal cell survival, differentiation and gene expression. Binding of ciliary neurotrophic factor to the encoded protein recruits the transmembrane components of the receptor, gp130 and leukemia inhibitory factor receptor, facilitating signal transduction. Single nucleotide polymorphisms in this gene may be associated with variations in muscle strength, as well as early onset of eating disorders. Alternatively spliced transcript variants have been observed for this gene.
Background References
1. Kim JW. et. al. Antitumor activity of an engineered decoy receptor targeting CLCF1-CNTFR signaling in lung adenocarcinoma. Nat Med. 2019 Nov
Subcellular Location
Cell membrane.
Synonyms
Ciliary neurotrophic factor receptor alpha antibody
Ciliary neurotrophic factor receptor antibody
Ciliary neurotrophic factor receptor subunit alpha antibody
CNTF Receptor alpha antibody
CNTF receptor subunit alpha antibody
CNTFR Alpha antibody
CNTFR antibody
CNTFR-alpha antibody
CNTFR_HUMAN antibody
Images
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Western blot analysis of CNTFR on different lysates with Rabbit anti-CNTFR antibody (HA500506) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate (20 ug/lane)
Lane 2: C6 cell lysate (20 ug/lane)
Lane 3: mouse brain tissue lysate (40 ug/lane)
Lane 4: rat brain tissue lysate (40 ug/lane)
Lane 5: mouse cerebellum tissue lysate (40 ug/lane)
Lane 6: rat cerebellum tissue lysate (40 ug/lane)
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500506, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 40.6 kDa
Observed band size: 60 kDa -
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CNTFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500506, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CNTFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500506, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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ICC staining of CNTFR in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500506, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of SH-SY5Y cells labeling CNTFR.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500506, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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