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DACT1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500500

DACT1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

DACT1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human DACT1 aa 637-836.

Species Reactivity

Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 84 kDa

Positive Control

Mouse liver tissue lysate, mouse testis tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue.

Conjugation

unconjugated

RRID

AB_3071585

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:600

Target

Function

The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene.

Background References

1. Hou J. et. al. DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43. Braz J Cardiovasc Surg. 2019 Dec

2. Yang JH. et. al. Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma. Biol Res. 2019 Jun

Subcellular Location

Cytoplasm, nucleus, synapse.

UNIPROT

SwissProt: Q9NYF0 Human

SwissProt: Q8R4A3 Mouse

Synonyms

Dact antibody

dact1 antibody

DACT1_HUMAN antibody

Dapper antagonist of beta catenin homolog 1 antibody

Dapper antagonist of catenin 1 antibody

Dapper antibody

Dapper homolog 1 antibody

DAPPER1 antibody

Dpr antibody

Dpr1 antibody

Expand

Images

  • Western blot analysis of DACT1 on different lysates with Rabbit anti-DACT1 antibody (<a href="/products/HA500500" style="font-weight: bold;text-decoration: underline;">HA500500</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Mouse testis tissue lysate<br />Lane 3: Mouse brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500500" style="font-weight: bold;text-decoration: underline;">HA500500</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 84 kDa<br />Observed band size: 100 kDa

    Western blot analysis of DACT1 on different lysates with Rabbit anti-DACT1 antibody (HA500500) at 1/5,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse testis tissue lysate
    Lane 3: Mouse brain tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500500, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 84 kDa
    Observed band size: 100 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DACT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500500" style="font-weight: bold;text-decoration: underline;">HA500500</a>, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DACT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500500, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"