DACT1 Rabbit Polyclonal Antibody
Catalog# HA500500
DACT1 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Mouse
-
unconjugated
Overview
Product Name
DACT1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human DACT1 aa 637-836.
Species Reactivity
Mouse
Validated Applications
WB, IHC-P
Molecular Weight
84 kDa
Positive Control
Mouse testis tissue lysate, mouse liver tissue lysate, mouse cerebellum tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
-
IHC-P
-
1:600
Target
Function
The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene.
Background References
1. Hou J. et. al. DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43. Braz J Cardiovasc Surg. 2019 Dec
2. Yang JH. et. al. Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma. Biol Res. 2019 Jun
Subcellular Location
Cytoplasm, nucleus, synapse.
Synonyms
Dact antibody
dact1 antibody
DACT1_HUMAN antibody
Dapper antagonist of beta catenin homolog 1 antibody
Dapper antagonist of catenin 1 antibody
Dapper antibody
Dapper homolog 1 antibody
DAPPER1 antibody
Dpr antibody
Dpr1 antibody
ExpandImages
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Western blot analysis of DACT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500500, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse testis tissue lysate
Lane 2: Mouse liver tissue lysate -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DACT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500500, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"