SOX17 Rabbit Polyclonal Antibody
Catalog# HA500495
SOX17 Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Mouse
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Rat
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unconjugated
Overview
Product Name
SOX17 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from Human SOX17 : 71-180/414
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 44 kDa
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
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IF-Tissue
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1:100-1:500
Target
Function
SOX17 is involved in the regulation of vertebrate embryonic development and in the determination of the endodermal cell fate. The encoded protein acts downstream of TGF beta signaling (Activin) and canonical WNT signaling (Wnt3a). Especially the correct phosphorylation of SMAD2/3 within the respective cell cycle (early G1 phase) is crucial for the activation of cardinal endodermal genes (e.g. SOX17) to further enter the definitive endodermal lineage. Besides that, perturbation of the SOX17 centromertic CTCF-boundary in early definitive endoderm differentiation, leads to massive developmental failure and a so-called mes-endodermal like trapped cell-state, which can be rescued by ectopic SOX17 expression. In Xenopus gastrulae it has been shown that SOX17 modifies Wnt responses, where genomic specificity of Wnt/β-catenin transcription is determined through functional interactions between SOX17 and β-catenin/Tcf transcriptional complexes.
Background References
1. Tan DS. et. al. SOX17 in cellular reprogramming and cancer. Semin Cancer Biol. 2020 Dec
2. Liu M. et. al. Sox17 is required for endothelial regeneration following inflammation-induced vascular injury. Nat Commun. 2019 May
Subcellular Location
Nucleus.
Synonyms
FLJ22252 antibody
SOX17 antibody
SOX17_HUMAN antibody
SRY (sex determining region Y) box 17 antibody
SRY box 17 antibody
SRY related HMG box transcription factor SOX17 antibody
Transcription factor SOX-17 antibody
Transcription factor SOX17 antibody
VUR3 antibody
Images
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Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Ovary tissue lysates
Primary: Anti-SOX17 (HA500495) at 1/1,000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa -
Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SOX17 Polyclonal Antibody, Unconjugated(HA500495) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"