WASP/Wiskott-Aldrich syndrome protein Rabbit Polyclonal Antibody
Catalog# HA500490
WASP/Wiskott-Aldrich syndrome protein Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
WASP/Wiskott-Aldrich syndrome protein Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human WASP aa 1-200.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 53 kDa
Positive Control
Rat bone marrow tissue lysates, Jurkat cell lysate, Daudi cell lysate, human spleen tissue, human tonsil tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:600
Target
Function
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.
Background References
1. Piperno GM. et. al. Wiskott-Aldrich syndrome protein restricts cGAS/STING activation by dsDNA immune complexes. JCI Insight. 2020 Sep
2. Rivers E. et. al. Wiskott-Aldrich syndrome protein: Emerging mechanisms in immunity. Eur J Immunol. 2017 Nov
Subcellular Location
Cytoskeleton, Nucleus.
Synonyms
Eczema thrombocytopenia antibody
IMD2 antibody
SCNX antibody
THC antibody
THC1 antibody
Thrombocytopenia 1 (X linked) antibody
U42471 antibody
Was antibody
WASp antibody
WASP_HUMAN antibody
ExpandImages
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Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500490, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 53 kDa
Observed band size: 60 kDa -
Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500490, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Daudi cell lysate
Predicted band size: 53 kDa
Observed band size: 60 kDa -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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