CDX4 Rabbit Polyclonal Antibody
Catalog# HA500489
CDX4 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CDX4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human CDX4 aa 50-100 / 284.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
31 kDa
Positive Control
Hela cell lysate, 293 cell lysate, rat cerebellum tissue, mouse testis tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
Target
Function
The transcription factor encoded by the CDX4 gene participates in the formation of extra-embryonic tissues, anterior-posterior patterning and blood formation during embryogenesis. It does so through the regulation of Hox gene expression. Before placentation takes place, CDX4 plays a role in its development. CDX4 mutants are born healthy and are fertile, however its importance is revealed in compound CDX mutants. Compound mutants carrying one CDX2 null allele and homozygous null for CDX4 fail to generate posterior tissue caudal to the hindlimbs and most of these embryos die around embryonic day 10.5 from lack of placental development. Around 10% of this phenotype may progress to full term, but then die shortly after birth. Upon inspection the morphogenesis of ano-rectal and urethral tissues was observed.
Background References
1. Alan J. Davidson. et. al. cdx4 mutants fail to specify blood progenitors and can be rescued by multiple hox genes. Nature volume 425, pages300–306(2003).
Subcellular Location
Nucleus.
Synonyms
Caudal-type homeobox protein 4 antibody
CDX4 antibody
CDX4_HUMAN antibody
Homeobox protein CDX-4 antibody
Images
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Western blot analysis of CDX4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500489, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CDX4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500489, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CDX4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500489, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"