SOX17 Rabbit Polyclonal Antibody
Catalog# HA500476
SOX17 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SOX17 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human SOX17 aa 1-50 / 414.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 44 kDa
Positive Control
SKOV-3 cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat brain tissue lysate, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:500-1:1,000
Target
Function
SOX17 is involved in the regulation of vertebrate embryonic development and in the determination of the endodermal cell fate. The encoded protein acts downstream of TGF beta signaling (Activin) and canonical WNT signaling (Wnt3a). Especially the correct phosphorylation of SMAD2/3 within the respective cell cycle (early G1 phase) is crucial for the activation of cardinal endodermal genes (e.g. SOX17) to further enter the definitive endodermal lineage. Besides that, perturbation of the SOX17 centromertic CTCF-boundary in early definitive endoderm differentiation, leads to massive developmental failure and a so-called mes-endodermal like trapped cell-state, which can be rescued by ectopic SOX17 expression. In Xenopus gastrulae it has been shown that SOX17 modifies Wnt responses, where genomic specificity of Wnt/β-catenin transcription is determined through functional interactions between SOX17 and β-catenin/Tcf transcriptional complexes.
Background References
1. Tan DS. et. al. SOX17 in cellular reprogramming and cancer. Semin Cancer Biol. 2020 Dec
2. Liu M. et. al. Sox17 is required for endothelial regeneration following inflammation-induced vascular injury. Nat Commun. 2019 May
Subcellular Location
Nucleus.
Synonyms
FLJ22252 antibody
SOX17 antibody
SOX17_HUMAN antibody
SRY (sex determining region Y) box 17 antibody
SRY box 17 antibody
SRY related HMG box transcription factor SOX17 antibody
Transcription factor SOX-17 antibody
Transcription factor SOX17 antibody
VUR3 antibody
Images
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Flow cytometric analysis of Hela cells labeling SOX17.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500476, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Western blot analysis of SOX17 on different lysates with Rabbit anti-SOX17 antibody (HA500476) at 1/1,000 dilution.
Lane 1: SK-OV-3 (Human ovarian cancer cells) cell lysate
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500476, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 44.1 kDa
Observed band size: 55 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"