CPN1 Rabbit Polyclonal Antibody
Catalog# HA500462
CPN1 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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Human
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Mouse
-
unconjugated
Overview
Product Name
CPN1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CPN1 aa 1-200 / 458.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell
Molecular Weight
52 kDa (Predicted band size)
Positive Control
Hela cell lysate, Raji cell lysate, mouse pancreas tissue lysate, LOVO.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
-
IF-Cell
-
1:50-1:200
Target
Function
Carboxypeptidase N catalytic chain is an enzyme that in humans is encoded by the CPN1 gene.Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer composed of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency.In melanocytic cells CPN1 gene expression may be regulated by MITF.
Background References
1. Keil C. et. al. Crystal structure of the human carboxypeptidase N (kininase I) catalytic domain. J. Mol. Biol. 366:504-516(2007).
Subcellular Location
Secreted.
Synonyms
ACBP antibody
Anaphylatoxin inactivator antibody
Arginine carboxypeptidase antibody
Carboxypeptidase N catalytic chain antibody
Carboxypeptidase N polypeptide 1 50 kD antibody
Carboxypeptidase N polypeptide 1 antibody
Carboxypeptidase N small subunit antibody
Carboxypeptidase N subunit 1 antibody
CBPN_HUMAN antibody
CPN antibody
ExpandImages
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Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500462, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Raji cell lysate
Lane 3: Mouse pancreas tissue lysate
Predicted band size: 52 kDa
Observed band size: 55 kDa -
ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500462, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"