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WDR77 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500456

WDR77 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

WDR77 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human WDR77 aa 1-342 / 342.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 37 kDa

Positive Control

Hep G2 cell lysate, Daudi cell lysate, Hela cell lysate, HEK-293 cell lysate, K-562 cell lysate, A549 cell lysate, Hela, HepG2.

Conjugation

unconjugated

RRID

AB_3071541

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:200

  • FC

  • 1:500-1:1,000

Target

Function

Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

Background References

1. Yuan H et al. HBx represses WDR77 to enhance HBV replication by DDB1-mediated WDR77 degradation in the liver. Theranostics. 2021 Jul

2. Zhao Y et al. Germ-line mutations in WDR77 predispose to familial papillary thyroid cancer. Proc Natl Acad Sci U S A. 2021 Aug

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9BQA1 Human

Synonyms

2610312E17Rik antibody

Androgen receptor cofactor p44 antibody

C79984 antibody

HKMT1069 antibody

MEP 50 antibody

MEP-50 antibody

MEP50 antibody

MEP50_HUMAN antibody

Methylosome protein 50 antibody

MGC2722 antibody

Expand

Images

  • Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (<a href="/products/HA500456" style="font-weight: bold;text-decoration: underline;">HA500456</a>) at 1/5,000 dilution.<br /><br />Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate<br />Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate<br />Lane 5: K-562 (Human chronic myelogenous leukemia cell) cell lysate<br />Lane 6: A549 (Human lung adenocarcinoma cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 6 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500456" style="font-weight: bold;text-decoration: underline;">HA500456</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 36.7 kDa<br />Observed band size: 40 kDa

    Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (HA500456) at 1/5,000 dilution.

    Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate
    Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate
    Lane 5: K-562 (Human chronic myelogenous leukemia cell) cell lysate
    Lane 6: A549 (Human lung adenocarcinoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500456, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 36.7 kDa
    Observed band size: 40 kDa

  • Immunocytochemistry analysis of Hela cells labeling WDR77 with Rabbit anti-WDR77 antibody (<a href="/products/HA500456" style="font-weight: bold;text-decoration: underline;">HA500456</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WDR77 antibody (<a href="/products/HA500456" style="font-weight: bold;text-decoration: underline;">HA500456</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling WDR77 with Rabbit anti-WDR77 antibody (HA500456) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WDR77 antibody (HA500456) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of HepG2 cells labeling WDR77.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500456" style="font-weight: bold;text-decoration: underline;">HA500456</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling WDR77.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500456, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of Hela cells labeling WDR77.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500456" style="font-weight: bold;text-decoration: underline;">HA500456</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Hela cells labeling WDR77.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500456, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

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WDR77 Recombinant Rabbit Monoclonal Antibody [PSH0-07]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated