JAZ11 Rabbit Polyclonal Antibody
Catalog# HA500450
JAZ11 Rabbit Polyclonal Antibody
-
ELISA
-
IF-Tissue
-
IHC-P
-
Rice
-
unconjugated
Overview
Product Name
JAZ11 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within rice JAZ11 aa 160-209 / 209.
Species Reactivity
Rice
Validated Applications
ELISA, IF-Tissue, IHC-P
Target Molecular Weight
22 kDa
Positive Control
Rice tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
ELISA
-
1:10,000
-
IF-Tissue
-
1:50-1:100
-
IHC-P
-
1:50-1:200
Target
Function
Repressor of jasmonate responses.
Background References
1. Song S. et. al. The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in Arabidopsis. Plant Cell. 2011 Mar;23(3):1000-13.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Protein TIFY 11c
OsTIFY11c
Jasmonate ZIM domain-containing protein 11
OsJAZ11
OsJAZ2
TIFY11C
JAZ11
Os03g0180900
LOC_Os03g08320
OsJ_09657
ExpandImages
-
Immunofluorescence staining of paraffin- embedded rice tissue using anti-JAZ11 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with JAZ11 antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
-
Immunohistochemical analysis of paraffin-embedded rice tissue using anti-JAZ11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500450, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"