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EGR4 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500445

EGR4 Rabbit Polyclonal Antibody

Application

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

EGR4 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within mouse EGR4 aa 300-478 / 589.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P

Target Molecular Weight

Predicted band size: 62 kDa

Positive Control

Human colon tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

RRID

AB_3071530

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:50-1:200

Target

Function

Early growth response protein 4 (EGR-4), also known as AT133, is a protein that in humans is encoded by the EGR4 gene. EGR-4 is a member of the early growth response (EGF) family of zinc finger transcription factors. Transcription factors EGR1, 2, and 3 also share an R1 domain, missing in EGR4, which can be bound by NAB proteins to prevent expression of EGR target genes. The EGR zinc finger motifs are designed to specifically recognize a 9 base pair consensus sequence (5′ GCG TGG GCG 3′), with each finger spanning three nucleotides. A loss of Egr4 results in a block in spermatogenesis at the early-mid pachytene spermatocyte stage leading to a significant reduction in the number of spermatozoa produced. Additionally, Erg4-null spermatozoa show abnormal morphology such as separated heads and fragmented flagellum. The block also occurred during the first wave of spermatogenesis as apoptotic germ cells were present from 14 dpp onward. The importance of EGR4 for fertility in mammals has also been demonstrated in human males with cryptorchidism.

Background References

1. Niu RN. et. al. Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway. Biochimie. 2018 Jun.

2. Sung SR. et. al. Sequence variations of the EGR4 gene in Korean men with spermatogenesis impairment. BMC Med Genet. 2017 May.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q05215 Human

SwissProt: Q9WUF2 Mouse

SwissProt: Q00911 Rat

Synonyms

AT133 antibody

Early growth response protein 4 antibody

Nerve growth factor induced clone C antibody

NGFI C antibody

NGFIC antibody

PAT133 antibody

Egr4

Images

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-EGR4 antibody (<a href="/products/HA500445" style="font-weight: bold;text-decoration: underline;">HA500445</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500445" style="font-weight: bold;text-decoration: underline;">HA500445</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-EGR4 antibody (HA500445) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EGR4 antibody (<a href="/products/HA500445" style="font-weight: bold;text-decoration: underline;">HA500445</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500445" style="font-weight: bold;text-decoration: underline;">HA500445</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EGR4 antibody (HA500445) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EGR4 antibody (<a href="/products/HA500445" style="font-weight: bold;text-decoration: underline;">HA500445</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500445" style="font-weight: bold;text-decoration: underline;">HA500445</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EGR4 antibody (HA500445) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"