RBR-type E3 ubiquitin transferase Rabbit Polyclonal Antibody
Catalog# HA500436
RBR-type E3 ubiquitin transferase Rabbit Polyclonal Antibody
-
WB
-
IF-Tissue
-
IHC-P
-
Rice
-
unconjugated
Overview
Product Name
RBR-type E3 ubiquitin transferase Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Rice RBR-type E3 ubiquitin transferase aa 31-70 / 543.
Species Reactivity
Rice
Validated Applications
WB, IF-Tissue, IHC-P
Target Molecular Weight
62 kDa
Positive Control
Rice tissue lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2000
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:200
Target
Function
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.
Background References
暂无
UNIPROT
Synonyms
RBR-type E3 ubiquitin transferas antibody
Images
-
Western blot analysis of RBR-type E3 ubiquitin transferase on rice tissue lysate . Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST. in PBS for 1 hour at room temperature. The primary antibody (HA500436, 1/500) was used in 5% NFDM/TBST. at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
-
Immunofluorescence staining of paraffin- embedded rice tissue using anti-RBR-type E3 ubiquitin transferase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody () at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
-
Immunohistochemical analysis of paraffin-embedded rice tissue using anti-RBR-type E3 ubiquitin transferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody () at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"