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PLCB2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500399

PLCB2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PLCB2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human PLCB2 aa 950-1185.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 134 kDa

Positive Control

THP-1 (Human acute monoblastic leukemia cell) cell lysate, HL-60.

Conjugation

unconjugated

RRID

AB_3071484

Product Features

Form

Liquid

Concentration

1.99 mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

Target

Function

1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 is an enzyme that in humans is encoded by the PLCB2 gene. The gene codes for the enzyme phospholipase C β2. The enzyme catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11, as well as G-beta gamma subunits. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses.

Background References

1. Tall E., Dorman G., Garcia P., Runnels L., Shah S., Chen J., Profit A., Gu Q.M., Chaudhary A., Prestwich G.D., Rebecchi M.J. Phosphoinositide binding specificity among phospholipase C isozymes as determined by photo-cross-linking to novel substrate and product analogs. Biochemistry 36:7239-7248(1997)

2. Park D., Jhon D.-Y., Kriz R., Knopf J., Rhee S.G. Cloning, sequencing, expression, and Gq-independent activation of phospholipase C-beta 2. J. Biol. Chem. 267:16048-16055(1992)

Subcellular Location

Cytosol.

UNIPROT

SwissProt: Q00722 Human

Synonyms

1-phosphatidylinositol-4 antibody

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 antibody

5-bisphosphate phosphodiesterase beta-2 antibody

FLJ38135 antibody

Phosphoinositide phospholipase C-beta-2 antibody

Phospholipase C-beta-2 antibody

PLC-beta-2 antibody

Plcb2 antibody

PLCB2_HUMAN antibody

Images

  • Western blot analysis of PLCB2 on THP-1 (Human acute monoblastic leukemia cell) cell lysate with Rabbit anti-PLCB2 antibody (<a href="/products/HA500399" style="font-weight: bold;text-decoration: underline;">HA500399</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 18 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500399" style="font-weight: bold;text-decoration: underline;">HA500399</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 134 kDa<br />Observed band size: 134 kDa

    Western blot analysis of PLCB2 on THP-1 (Human acute monoblastic leukemia cell) cell lysate with Rabbit anti-PLCB2 antibody (HA500399) at 1/5,000 dilution.

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 18 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500399, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 134 kDa
    Observed band size: 134 kDa

  • Immunocytochemistry analysis of HL-60 cells labeling PLCB2 with Rabbit anti-PLCB2 antibody (<a href="/products/HA500399" style="font-weight: bold;text-decoration: underline;">HA500399</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB2 antibody (<a href="/products/HA500399" style="font-weight: bold;text-decoration: underline;">HA500399</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HL-60 cells labeling PLCB2 with Rabbit anti-PLCB2 antibody (HA500399) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB2 antibody (HA500399) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"