PLCB3 Rabbit Polyclonal Antibody
Catalog# HA500391
PLCB3 Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PLCB3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PLCB3 aa 2-251.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, FC
Target Molecular Weight
Predicted band size: 139 kDa
Positive Control
NIH/3T3 cell lysates, human small intestine tissue, rat cerebellum tissue, HepG2, PC-12.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
2.06 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
-
1:600
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IF-Cell
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1:100-1:200
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FC
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1:1,000
Target
Function
1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3 is an enzyme that in humans is encoded by the PLCB3 gene. The gene codes for the enzyme phospholipase C β3. The enzyme catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11, as well as G-beta gamma subunits. PLCB3 has been shown to interact with Sodium-hydrogen exchange regulatory cofactor 2.
Background References
1. Hwang JI, Heo K, Shin KJ, Kim E, Yun C, Ryu SH, Shin HS, Suh PG (June 2000). "Regulation of phospholipase C-beta 3 activity by Na+/H+ exchanger regulatory factor 2". J. Biol. Chem. 275 (22): 16632–7.
2. Ben-Salem S., Robbins S.M., Lm Sobreira N., Lyon A., Al-Shamsi A.M., Islam B.K., Akawi N.A., John A., Thachillath P., Al Hamed S., Valle D., Ali B.R., Al-Gazali L. Defect in phosphoinositide signalling through a homozygous variant in PLCB3 causes a new form of spondylometaphyseal dysplasia with corneal dystrophy. J. Med. Genet. 55:122-130(2018)
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
1-phosphatidylinositol-4 antibody
5-bisphosphate phosphodiesterase beta-3 antibody
Phosphoinositide phospholipase C antibody
Phosphoinositide phospholipase C-beta-3 antibody
Phospholipase C beta 3 antibody
Phospholipase C beta 3 (phosphatidylinositol specific) antibody
Phospholipase C-beta-3 antibody
PLC beta 3 antibody
PLC-beta-3 antibody
ExpandImages
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Western blot analysis of PLCB3 on NIH/3T3 cell lysate with Rabbit anti-PLCB3 antibody (HA500391) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 180 seconds ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500391, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 139 kDa
Observed band size: 150 kDa -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HepG2 cells labeling PLCB3 with Rabbit anti-PLCB3 antibody (HA500391) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB3 antibody (HA500391) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling PLCB3 with Rabbit anti-PLCB3 antibody (HA500391) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLCB3 antibody (HA500391) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of PC-12 cells labeling PLCB3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500391, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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