RBBP5 Rabbit Polyclonal Antibody
Catalog# HA500389
RBBP5 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
RBBP5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human RBBP5 aa 1-50.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 59 kDa
Positive Control
MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, human lung carcinoma tissue, human colon tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
2.1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:600
Target
Function
In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Background References
1. Avdic V., Zhang P., Lanouette S., Groulx A., Tremblay V., Brunzelle J., Couture J.F. Structural and biochemical insights into MLL1 core complex assembly. Structure 19:101-108(2011).
2. Zhang P., Lee H., Brunzelle J.S., Couture J.F. The plasticity of WDR5 peptide-binding cleft enables the binding of the SET1 family of histone methyltransferases. Nucleic Acids Res. 40:4237-4246(2012).
Subcellular Location
Nucleus.
Synonyms
RBBP 5 antibody
RBBP-5 antibody
RBBP5 antibody
RBBP5_HUMAN antibody
RBQ 3 antibody
RBQ3 antibody
Retinoblastoma binding protein 5 antibody
Retinoblastoma binding protein RBQ3 antibody
Retinoblastoma binding protein5 antibody
Retinoblastoma-binding protein 5 antibody
ExpandImages
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Western blot analysis of RBBP5 on different lysates with Rabbit anti-RBBP5 antibody (HA500389) at 1/2,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500389, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 59 kDa
Observed band size: 59 kDa -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-RBBP5 antibody (HA500389) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500389) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RBBP5 antibody (HA500389) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500389) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
