PSME4 Rabbit Polyclonal Antibody
Catalog# HA500375
PSME4 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PSME4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PSME4 aa 1,550-1,843.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 211 kDa
Positive Control
293T cell lysate, Hela cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, rat skeletal muscle tissue lysate, rat liver tissue, mouse liver tissue.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1.57 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
Background References
1. Blickwedehl J., Olejniczak S., Cummings R., Sarvaiya N., Mantilla A., Chanan-Khan A., Pandita T.K., Schmidt M., Thompson C.B., Bangia N. The proteasome activator PA200 regulates tumor cell responsiveness to glutamine and resistance to ionizing radiation. Mol. Cancer Res. 10:937-944(2012).
2. Blickwedehl J., Agarwal M., Seong C., Pandita R.K., Melendy T., Sung P., Pandita T.K., Bangia N. Role for proteasome activator PA200 and postglutamyl proteasome activity in genomic stability. Proc. Natl. Acad. Sci. U.S.A. 105:16165-16170(2008).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
KIAA0077 antibody
PA200 antibody
Proteasome Activator 200 kDa antibody
Proteasome activator complex subunit 4 antibody
Proteasome activator PA200 antibody
PSME 4 antibody
psme4 antibody
PSME4_HUMAN antibody
Images
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Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (HA500375) at 1/500 dilution.
Lane 1: 293T cell lysate (10 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Lane 3: Jurkat cell lysate (10 µg/Lane)
Lane 4: RAW264.7 cell lysate (10 µg/Lane)
Lane 5: Rat skeletal muscle tissue lysate (20 µg/Lane)
Predicted band size: 211 kDa
Observed band size: 211 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500375) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"