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TMED5 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500347

TMED5 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TMED5 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human TMED5 aa 1-200 / 229.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 26 kDa

Positive Control

HeLa cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse liver tissue lysate, rat bladder tissue, mouse large intestine tissue.

Conjugation

unconjugated

RRID

AB_3071433

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IF-Cell
human
Tested Tested
Not Recommended
mouse
Tested Tested
Not Recommended
rat
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:100,000

  • IHC-P

  • 1:600-1:5,000

Target

Function

Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Required for the maintenance of the Golgi apparatus; involved in protein exchange between Golgi stacks during assembly. Probably not required for COPI-vesicle-mediated retrograde transport. TMED5 is a part of the p24 protein family whose general functions are protein trafficking for the secretory pathway. TMED5 is thought to be necessary in the formation of the Golgi into a ribbon. Glycosylphosphatidylinositol-anchored proteins (GPI-AP) depend on p24 cargo receptors for transport from the ER to the Golgi. Knockdown of p24γ2 (a mouse ortholog of TMED5) in mice resulted in impaired transport of GPI-AP. The study concluded that the α-helical region of p24γ2 binds GPI which is necessary to incorporate it into COPII transport vesicles. TMED5 is reported to be necessary for the secretion of Wnt ligands. TMED5 has been found to interact with WNT7B, activating the canonical WNT-CTNNB1/β-catenin signaling pathway. This pathway is linked to numerous cancers because upregulation of the Wnt/β-catenin signaling pathway leads to cytosolic accumulation of β-catenin, promoting cellular proliferation.

Background References

1. Bao C. et. al. TP73-AS1 promotes gastric cancer proliferation and invasion by regulation miR-27b-3p/TMED5 axis. J Cancer. 2022 Feb

2. Zou H. et. al. Identification of a novel circ_0018289/miR-183-5p/TMED5 regulatory network in cervical cancer development. World J Surg Oncol. 2021 Aug

Subcellular Location

Cis-Golgi network membrane, endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane.

UNIPROT

SwissProt: Q9Y3A6 Human

SwissProt: Q9CXE7 Mouse

SwissProt: Q6AXN3 Rat

Synonyms

CGI 100 antibody

DKFZp781B2248 antibody

p24 family protein gamma-2 antibody

p24gamma2 antibody

p28 antibody

RP5 976O13.2 antibody

TMED 5 antibody

TMED5 antibody

TMED5_HUMAN antibody

Transmembrane emp24 domain containing protein 5 antibody

Expand

Images

  • Western blot analysis of TMED5 on different lysates with Rabbit anti-TMED5 antibody (<a href="/products/HA500347" style="font-weight: bold;text-decoration: underline;">HA500347</a>) at 1/100,000 dilution.<br /><br />Lane 1: HeLa cell lysate (10 µg/Lane)<br />Lane 2: RAW264.7 cell lysate (10 µg/Lane)<br />Lane 3: C6 cell lysate (10 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (10 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500347" style="font-weight: bold;text-decoration: underline;">HA500347</a>) at 1/100,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMED5 on different lysates with Rabbit anti-TMED5 antibody (HA500347) at 1/100,000 dilution.

    Lane 1: HeLa cell lysate (10 µg/Lane)
    Lane 2: RAW264.7 cell lysate (10 µg/Lane)
    Lane 3: C6 cell lysate (10 µg/Lane)
    Lane 4: Mouse liver tissue lysate (10 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500347) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-TMED5 antibody (<a href="/products/HA500347" style="font-weight: bold;text-decoration: underline;">HA500347</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500347" style="font-weight: bold;text-decoration: underline;">HA500347</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-TMED5 antibody (<a href="/products/HA500347" style="font-weight: bold;text-decoration: underline;">HA500347</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500347" style="font-weight: bold;text-decoration: underline;">HA500347</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"