GRB7 Rabbit Polyclonal Antibody
Catalog# HA500328
GRB7 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
GRB7 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human GRB7 aa 1-200/532.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 60 kDa
Positive Control
SK-Br-3 cell lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
Target
Function
Growth factor receptor-bound protein 7, also known as GRB7, is a protein that in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular direct link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7, thought to be involved in migration[citation needed], is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.
Background References
1. Gotovac JR. et. al. GRB7 is an oncogenic driver and potential therapeutic target in oesophageal adenocarcinoma. J Pathol. 2020 Nov
2. Zheng Y. et. al. Upregulated GRB7 promotes proliferation and tumorigenesis of Bladder Cancer via Phospho-AKT Pathway. Int J Biol Sci. 2020 Oct
Subcellular Location
Cell membrane, Cytoplasm, Focal adhesion, Cytoplasmic granule, Cell projection.
UNIPROT
Synonyms
B47 antibody
Epidermal growth factor receptor GRB 7 antibody
Epidermal growth factor receptor GRB-7 antibody
GRB7 adapter protein antibody
GRB7 antibody
GRB7_HUMAN antibody
Growth factor receptor bound protein 7 antibody
Growth factor receptor-bound protein 7 antibody
OTTHUMP00000164349 antibody
OTTHUMP00000164350 antibody
ExpandImages
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Western blot analysis of GRB7 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500328, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GRB7 on SK-Br-3 cell lysates with Rabbit anti-GRB7 antibody (HA500328) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500328) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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