BMP10 Rabbit Polyclonal Antibody
Catalog# HA500312
BMP10 Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
BMP10 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human BMP10 aa 100-310 / 424.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
NCI-H1299 cell lysate, MCF7 cell lysate, 293T cell lysate.
Conjugation
unconjugated
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines.
Background References
1. Mazerbourg S. et. al. Identification of receptors and signaling pathways for orphan bone morphogenetic protein/growth differentiation factor ligands based on genomic analyses. J. Biol. Chem. 280:32122-32132(2005).
Subcellular Location
Secreted.
UNIPROT
Synonyms
BMP-10 antibody
Bmp10 antibody
BMP10_HUMAN antibody
Bone morphogenetic protein 10 antibody
Bone morphogenetic protein 10 preproprotein antibody
MGC126783 antibody
OTTHUMP00000159973 antibody
Images
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Western blot analysis of BMP10 on different lysates with Rabbit anti-BMP10 antibody (HA500312) at 1/2,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500312) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"