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ATP1B4 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500311

ATP1B4 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ATP1B4 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human ATP1B4 100-300 / 357.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, Mouse heart tissue lysate, Human skeletal muscle tissue lysate, Mouse tongue tissue lysate, 293 cell lysate.

Conjugation

unconjugated

RRID

AB_3071406

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

May act as a transcriptional coregulator during muscle development through its interaction with SNW1. Has lost its ancestral function as a Na,K-ATPase beta-subunit.

Background References

1. Pestov N.B. et. al. Evolution of Na,K-ATPase betam-subunit into a coregulator of transcription in placental mammals. Proc. Natl. Acad. Sci. U.S.A. 104:11215-11220(2007).

Subcellular Location

Membrane, Nucleus.

UNIPROT

SwissProt: Q9UN42 Human

SwissProt: Q99ME6 Mouse

SwissProt: Q9R193 Rat

Synonyms

Protein ATP1B4 antibody

X,K-ATPase subunit beta-m antibody

X/potassium-transporting ATPase subunit beta-m antibody

ATP1B4 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ATP1B4 on different lysates with Rabbit anti-ATP1B4 antibody (<a href="/products/HA500311" style="font-weight: bold;text-decoration: underline;">HA500311</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse skeletal muscle tissue lysate<br />Lane 2: Rat skeletal muscle tissue lysate<br />Lane 3: Mouse heart tissue lysate (low expression)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500311" style="font-weight: bold;text-decoration: underline;">HA500311</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ATP1B4 on different lysates with Rabbit anti-ATP1B4 antibody (HA500311) at 1/2,000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate
    Lane 3: Mouse heart tissue lysate (low expression)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 8 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500311) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ATP1B4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500311" style="font-weight: bold;text-decoration: underline;">HA500311</a>, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Human skeletal muscle tissue lysate<br />Lane 2: Mouse skeletal muscle tissue lysate<br />Lane 3: Mouse tongue tissue lysate<br />Lane 4: 293 cell lysate

    Western blot analysis of ATP1B4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500311, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Human skeletal muscle tissue lysate
    Lane 2: Mouse skeletal muscle tissue lysate
    Lane 3: Mouse tongue tissue lysate
    Lane 4: 293 cell lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"