p114RhoGEF Rabbit Polyclonal Antibody
Catalog# HA500310
p114RhoGEF Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
p114RhoGEF Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human p114RhoGEF aa 1000-1200 / 1361.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 152 kDa
Positive Control
HepG2 cell lysate, Hela cell lysate, 293 cell lysate, PC-3 cell lysate, Daudi cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background References
1. Turton KB. et. al. Expression of novel "LOCGEF" isoforms of ARHGEF18 in eosinophils. J Leukoc Biol. 2018 Jul
2. Arno G. et. al. Biallelic Mutation of ARHGEF18, Involved in the Determination of Epithelial Apicobasal Polarity, Causes Adult-Onset Retinal Degeneration. Am J Hum Genet. 2017 Feb
Subcellular Location
Cytoskeleton, Cell membrane, Apical cell membrane, Cytoplasm.
Synonyms
114 kDa Rho specific guanine nucleotide exchange factor antibody
114 kDa Rho-specific guanine nucleotide exchange factor antibody
ARHGEF 18 antibody
Arhgef18 antibody
ARHGI_HUMAN antibody
KIAA0521 antibody
MGC15913 antibody
p114 Rho GEF antibody
p114-Rho-GEF antibody
p114RhoGEF antibody
ExpandImages
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Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500310, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: 293 cell lysate
Lane 4: PC-3 cell lysate
Lane 5: Daudi cell lysate
Predicted band size: 152 kDa
Observed band size: 114/152 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"