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p114RhoGEF Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500310

p114RhoGEF Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

p114RhoGEF Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human p114RhoGEF aa 1000-1200 / 1361.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 152 kDa

Positive Control

HepG2 cell lysate, Hela cell lysate, 293 cell lysate, PC-3 cell lysate, Daudi cell lysate.

Conjugation

unconjugated

RRID

AB_3071405

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified.

Background References

1. Turton KB. et. al. Expression of novel "LOCGEF" isoforms of ARHGEF18 in eosinophils. J Leukoc Biol. 2018 Jul

2. Arno G. et. al. Biallelic Mutation of ARHGEF18, Involved in the Determination of Epithelial Apicobasal Polarity, Causes Adult-Onset Retinal Degeneration. Am J Hum Genet. 2017 Feb

Subcellular Location

Cytoskeleton, Cell membrane, Apical cell membrane, Cytoplasm.

UNIPROT

SwissProt: Q6ZSZ5 Human

SwissProt: F1LT94 Rat

Synonyms

114 kDa Rho specific guanine nucleotide exchange factor antibody

114 kDa Rho-specific guanine nucleotide exchange factor antibody

ARHGEF 18 antibody

Arhgef18 antibody

ARHGI_HUMAN antibody

KIAA0521 antibody

MGC15913 antibody

p114 Rho GEF antibody

p114-Rho-GEF antibody

p114RhoGEF antibody

Expand

Images

  • Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500310" style="font-weight: bold;text-decoration: underline;">HA500310</a>, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: HepG2 cell lysate<br />Lane 2: Hela cell lysate<br />Lane 3: 293 cell lysate<br />Lane 4: PC-3 cell lysate<br />Lane 5: Daudi cell lysate<br /><br />Predicted band size: 152 kDa<br />Observed band size: 114/152 kDa

    Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500310, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: 293 cell lysate
    Lane 4: PC-3 cell lysate
    Lane 5: Daudi cell lysate

    Predicted band size: 152 kDa
    Observed band size: 114/152 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"