TAZ Rabbit Polyclonal Antibody
Catalog# HA500300
TAZ Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
TAZ Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human TAZ aa 1-200 / 400.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
44 kDa
Positive Control
Human placenta tissue lysate, 293T cell lysate, human kidney tissue, mouse brain tissue, A549, HT-29.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:100-1:500
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IF-Cell
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1:100-1:200
Target
Function
Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis . The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ . WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation . In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation . Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
Background References
1. Kanai F. et. al. TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins. EMBO J. 19:6778-6791(2000).
Subcellular Location
Cell membrane, Cytoplasm, Membrane, Nucleus.
Synonyms
DKFZP586I1419 antibody
FLJ27004 antibody
FLJ45718 antibody
OTTHUMP00000215994 antibody
OTTHUMP00000215995 antibody
OTTHUMP00000215996 antibody
OTTHUMP00000216001 antibody
TAZ antibody
Transcriptional co activator with PDZ binding motif antibody
Transcriptional coactivator with PDZ binding motif antibody
ExpandImages
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Western blot analysis of TAZ on different lysates with Rabbit anti-TAZ antibody (HA500300) at 1/5,000 dilution.
Lane 1: HeLa
Lane 2: PANC-1
Lane 3: SH-SY5Y
Lane 4: SK-MEL-28
Lane 5: Raji
Lane 6: A375
Lysates/proteins at 20 µg/Lane.
Exposure time: 15 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500300, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 44.1 kDa
Observed band size: 52 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TAZ antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500300, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TAZ antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500300, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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ICC staining of TAZ in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500300, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of TAZ in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500300, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
TAZ / WWTR1 Recombinant Rabbit Monoclonal Antibody [PSH15-58]
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
