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HLA E Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500299

HLA E Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

HLA E Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human HLA E aa 22-200 / 358 (Extracellular).

Species Reactivity

Human

Validated Applications

WB, FC

Target Molecular Weight

40 kDa

Positive Control

SiHa, A549 cell lysate, A431 cell lysate.

Conjugation

unconjugated

RRID

AB_3071394

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:100-1:500

Target

Function

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

Background References

1. Grant EJ. et. al. The unconventional role of HLA-E: The road less traveled. Mol Immunol. 2020 Apr

2. Kanevskiy L. et. al. Dimorphism of HLA-E and its Disease Association. Int J Mol Sci. 2019 Nov

Subcellular Location

Golgi apparatus membrane, Cell membrane, Secreted.

UNIPROT

SwissProt: P13747 Human

Synonyms

HLA class I histocompatibility antigen E alpha chain antibody

EA1.2 antibody

EA2.1 antibody

HLA 6.2 antibody

HLA class I histocompatibility antigen alpha chain E antibody

HLA class I histocompatibility antigen E alpha chain precursor antibody

HLA class I histocompatibility antigen E alpha chain precursor antibody

HLA6.2 antibody

HLAE antibody

Lymphocyte antigen antibody

Expand

Images

  • Flow cytometric analysis of HLA E was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA500299" style="font-weight: bold;text-decoration: underline;">HA500299</a>, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HLA E was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500299, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of HLA E on different lysates with Rabbit anti-HLA E antibody (<a href="/products/HA500299" style="font-weight: bold;text-decoration: underline;">HA500299</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate <br />Lane 2: A431 cell lysate <br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500299" style="font-weight: bold;text-decoration: underline;">HA500299</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa

    Western blot analysis of HLA E on different lysates with Rabbit anti-HLA E antibody (HA500299) at 1/1,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: A431 cell lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500299, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"