GABRA2 Rabbit Polyclonal Antibody
Catalog# HA500288
GABRA2 Rabbit Polyclonal Antibody
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WB
-
FC
-
Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
GABRA2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human GABRA2 aa 29-200 / 451 (Extracellular).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
Rat brain tissue lysates, mouse brain tissue lysate, mouse cerebellum tissue lysate, human brain tissue lysate, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:20,000
-
FC
-
1:500-1:1,000
Target
Function
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Yu W. et. al. Gabra2 is a genetic modifier of Scn8a encephalopathy in the mouse. Epilepsia. 2020 Dec
2. Goetjen A. et. al. Induced pluripotent stem cell reprogramming-associated methylation at the GABRA2 promoter and chr4p12 GABA(A) subunit gene expression in the context of alcohol use disorder. Am J Med Genet B Neuropsychiatr Genet. 2020 Dec
Subcellular Location
Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Dendrite.
Synonyms
GABA A receptor subunit alpha 2 antibody
GABA antibody
GABA(A) receptor subunit alpha 2 antibody
GABA(A) receptor subunit alpha-2 antibody
GABR A2 antibody
GABRA 2 antibody
GABRA2 antibody
GABRA2 protein antibody
Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
Gamma aminobutyric acid A receptor alpha 2 antibody
ExpandImages
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Western blot analysis of GABRA2 on different lysates with Rabbit anti-GABRA2 antibody (HA500288) at 1/20,000 dilution.
Lane 1: Mouse brain(Unboiled) tissue lysate
Lane 2: Mouse lung(Unboiled) tissue lysate(negative)
Lane 3: Rat brain(Unboiled) tissue lysate
Lane 4: Rat lung(Unboiled) tissue lysate(negative)
Lysates/proteins at 20 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500288, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Flow cytometric analysis of Hela cells labeling GABRA2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500288, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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