TIFY11A Rabbit Polyclonal Antibody
Catalog# HA500265
TIFY11A Rabbit Polyclonal Antibody
-
WB
-
ELISA
-
Oryza sativa
-
unconjugated
Overview
Product Name
TIFY11A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within rice TIFY11A aa 50-179/179.
Species Reactivity
Oryza sativa
Validated Applications
WB, ELISA
Molecular Weight
18 kDa
Positive Control
Rice tissue lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Repressor of jasmonate (JA) responses. Forms a ternary complex with RSS3 and BHLH94 to negatively regulate JA-responsive genes. Acts as positive regulator of tolerance to salt stress. Involved in salt tolerance by modulating potassium homeostasis through JA signaling and regulation of the expression of potassium ion transporter genes. Acts as a transcriptional regulator targeted by the SCF(COI1) E3 ubiquitin ligase complexes in the JA signaling pathway, and interacts with BHLH062 that may directly regulate the ion transporter genes. Acts as positive regulator of tolerance to dehydration stress. Acts as negative regulator of tolerance to cold stress by interacting with MYB30.
Background References
1. Lv Y. et. al. The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing beta-amylase expression. Plant Physiol. 173:1475-1491(2017)
2. Ye H. et. al. Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice. Plant Mol. Biol. 71:291-305(2009)
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Protein TIFY 11a
OsTIFY11a
Jasmonate ZIM domain-containing protein 9
OsJAZ9
OsJAZ3
TIFY11A
JAZ9
Os03g0180800
LOC_Os03g08310
OsJ_09656
ExpandImages
-
Western blot analysis of TIFY11A on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500265, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"