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Pancreatic triacylglycerol lipase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500257

Pancreatic triacylglycerol lipase Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Pancreatic triacylglycerol lipase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Pancreatic triacylglycerol lipase aa 1-200 / 465.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

Mouse pancreas tissue lysate, rat pancreas tissue lysate, rat pancreas tissue, A549, SiHa.

Conjugation

unconjugated

RRID

AB_3071353

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:20,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:500-1:1,000

Target

Function

Pancreatic lipase, also known as pancreatic triacylglycerol lipase or steapsin, is an enzyme secreted from the pancreas. As the primary lipase enzyme that hydrolyzes (breaks down) dietary fat molecules in the human digestive system, it is one of the main digestive enzymes, converting triglyceride substrates like 1 found in ingested oils to monoglycerides 3 and free fatty acids 2a and 2b.Bile salts secreted from the liver and stored in gallbladder are released into the duodenum, where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

Background References

1. Szabo A. et. al. A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding. Biochim. Biophys. Acta 1852:1372-1379(2015).

Subcellular Location

Secreted.

UNIPROT

SwissProt: P16233 Human

SwissProt: Q6P8U6 Mouse

SwissProt: P27657 Rat

Synonyms

lipase, pancreatic antibody

LIPP_HUMAN antibody

Pancreatic lipase antibody

Pancreatic triacylglycerol lipase antibody

PL antibody

PNLIP antibody

PNLIPD antibody

PTL antibody

Triacylglycerol acylhydrolase antibody

Images

  • Western blot analysis of Pancreatic triacylglycerol lipase on different lysates with Rabbit anti-Pancreatic triacylglycerol lipase antibody (<a href="/products/HA500257" style="font-weight: bold;text-decoration: underline;">HA500257</a>) at 1/20,000 dilution.<br /><br />Lane 1: Mouse pancreas tissue lysate<br />Lane 2: Rat pancreas tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 45/51 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500257" style="font-weight: bold;text-decoration: underline;">HA500257</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Pancreatic triacylglycerol lipase on different lysates with Rabbit anti-Pancreatic triacylglycerol lipase antibody (HA500257) at 1/20,000 dilution.

    Lane 1: Mouse pancreas tissue lysate
    Lane 2: Rat pancreas tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 45/51 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500257) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Pancreatic triacylglycerol lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500257" style="font-weight: bold;text-decoration: underline;">HA500257</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Pancreatic triacylglycerol lipase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500257, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Pancreatic triacylglycerol lipase was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/HA500257" style="font-weight: bold;text-decoration: underline;">HA500257</a>, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Pancreatic triacylglycerol lipase was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500257, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • ICC staining of Pancreatic triacylglycerol lipase in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA500257" style="font-weight: bold;text-decoration: underline;">HA500257</a>, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Pancreatic triacylglycerol lipase in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500257, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"