IL-10RA Rabbit Polyclonal Antibody
Catalog# HA500251
IL-10RA Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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Human
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unconjugated
Overview
Product Name
IL-10RA Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL-10RA aa 491-540/578.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
63 kDa
Positive Control
Daudi cell lysates, Jurkat cell lysate, THP-1 cell lysate, MCF-7 cell lysate, human tonsil tissue, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:50-1:200
Target
Function
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
Background References
1. Prokoph N. et. al. IL10RA modulates crizotinib sensitivity in NPM1-ALK+ anaplastic large cell lymphoma. Blood. 2020 Oct
2. Xue AJ. et. al. Intestinal dysbiosis in pediatric Crohn\'s disease patients with IL10RA mutations. World J Gastroenterol. 2020 Jun
Subcellular Location
Cytoplasm, Cell membrane.
UNIPROT
Synonyms
CD210 antibody
CDw210a antibody
CDw210a antigen antibody
HIL 10R antibody
HIL10R antibody
I10R1_HUMAN antibody
IL 10R 1 antibody
IL 10R A antibody
IL 10R antibody
IL 10R1 antibody
ExpandImages
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Western blot analysis of IL-10RA on MCF7 (Human breast cancer cell) cell lysate with Rabbit anti-IL-10RA antibody (HA500251) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 90 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500251, 1/1,000 in5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-10RA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500251, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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ICC staining of IL-10RA in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500251, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"