BTNL3 Rabbit Polyclonal Antibody
Catalog# HA500239
BTNL3 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
BTNL3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human BTNL3 aa 150-200.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
Jurkat cell lysate, K562 cell lysate, HL-60 cell lysate, human spleen tissue, human small intestine tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:100-1:500
Target
Function
Butyrophilin-like 3 (BTNL3) is a member of the BTN/MOG Ig-superfamily which is coded by the BTNL3 gene, also known as COLF4100. BTNL3 is a 466 amino acid (aa) type I transmembrane protein that contains a 220 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 208 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in BTN/BTNL proteins, but the IgC domain is relatively weak. The ECD of human BTNL3 shares only 37% and 34% aa sequence identity with mouse (referred to as BTNL1) and rat, respectively. Human BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils. BTNL3 expression has also been found to be down-regulated in colon cancer tumors. The specific function of BTNL3, however, has yet to be fully elucidated. R&D in house data indicate that BTNL3 protein inhibits the secretion of IFN-gamma from anti-CD3 activated human T cells.
Background References
1. Shibui A. et. al. Cloning, expression analysis, and chromosomal localization of a novel butyrophilin-like receptor. J. Hum. Genet. 44:249-252(1999)
Subcellular Location
Membrane.
UNIPROT
Synonyms
BTNL3 antibody
BTNL3_HUMAN antibody
BTNLR antibody
Butyrophilin like protein 3 antibody
Butyrophilin like receptor antibody
Butyrophilin-like protein 3 antibody
Butyrophilin-like receptor antibody
COLF4100 antibody
Images
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Western blot analysis of BTNL3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500239, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 3: HL-60 cell lysate -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BTNL3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500239, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-BTNL3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500239, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"