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SFT Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500229

SFT Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SFT Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human SFT aa 1-147.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 17 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse testis tissue lysate, rat skeletal muscle tissue lysate, rat kidney tissue, human breast tissue, mouse skeletal muscle tissue.

Conjugation

unconjugated

RRID

AB_3071326

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:400

Target

Function

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.

Background References

1. Zhou C. et. al. Gain of UBE2D1 facilitates hepatocellular carcinoma progression and is associated with DNA damage caused by continuous IL-6. J Exp Clin Cancer Res. 2018 Nov

2. Hou L. et. al. UBE2D1 RNA Expression Was an Independent Unfavorable Prognostic Indicator in Lung Adenocarcinoma, but Not in Lung Squamous Cell Carcinoma. Dis Markers. 2018 Oct

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P51668 Human

SwissProt: P61080 Mouse

SwissProt: D3ZDK2 Rat

Synonyms

E2(17)KB1 antibody

SFT antibody

Stimulator of Fe transport antibody

UB2D1_HUMAN antibody

UBC 4/5 antibody

UBC4/5 antibody

UBC4/5 homolog antibody

UBC4/5, S. cerevisiae, homolog of antibody

UBCH 5 antibody

UBCH 5A antibody

Expand

Images

  • Western blot analysis of SFT on different lysates with Rabbit anti-SFT antibody (<a href="/products/HA500229" style="font-weight: bold;text-decoration: underline;">HA500229</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (10 µg/Lane)<br />Lane 2: Jurkat cell lysate (10 µg/Lane)<br />Lane 3: Raji cell lysate (10 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (10 µg/Lane)<br />Lane 5: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 6: Mouse testis tissue lysate (20 µg/Lane)<br />Lane 7: Rat skeletal muscle tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 17 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500229" style="font-weight: bold;text-decoration: underline;">HA500229</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SFT on different lysates with Rabbit anti-SFT antibody (HA500229) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (10 µg/Lane)
    Lane 2: Jurkat cell lysate (10 µg/Lane)
    Lane 3: Raji cell lysate (10 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
    Lane 5: Mouse liver tissue lysate (20 µg/Lane)
    Lane 6: Mouse testis tissue lysate (20 µg/Lane)
    Lane 7: Rat skeletal muscle tissue lysate (20 µg/Lane)

    Predicted band size: 17 kDa
    Observed band size: 15 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500229) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500229" style="font-weight: bold;text-decoration: underline;">HA500229</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500229" style="font-weight: bold;text-decoration: underline;">HA500229</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500229" style="font-weight: bold;text-decoration: underline;">HA500229</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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SFT Recombinant Rabbit Monoclonal Antibody [JE64-46]

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Products with the same target and pathway

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SFT Recombinant Rabbit Monoclonal Antibody [JE64-46]

Application: WB,IHC-P,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated