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NCOR2/SMRT Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500226

NCOR2/SMRT Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Mouse

  • Rat

Predicted reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NCOR2/SMRT Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within N-terminal human NCOR2.

Species Reactivity

Mouse, Rat (Predicted: Human)

Predicted species support after-sales service

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 274 kDa

Positive Control

Mouse brain tissue lysate, rat brain tissue lysate, MG-63.

Conjugation

unconjugated

RRID

AB_3071323

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

SMRT / NCoR2 (Nuclear receptor corepressor 2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, Thyroid and retinoic-acid-receptor-associated corepressor, TRAC) is a transcriptional corepressor for nuclear receptors. SMRT / NCoR2 has been shown to play a role in regulating immune response, mediating DNA repair, and in neuronal differentiation and cardiac development. SMRT / NCoR2 is part of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2.

Background References

1. Sharma N. et. al. ARNT2 Tunes Activity-Dependent Gene Expression through NCoR2-Mediated Repression and NPAS4-Mediated Activation. Neuron. 2019 Apr

2. Zhou W. et. al. Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection. Nat Neurosci. 2019 Feb

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9Y618 Human

SwissProt: Q9WU42 Mouse

SwissProt: D3ZXN9 Rat

Synonyms

CTG 26 antibody

CTG repeat protein 26 antibody

CTG26 antibody

N CoR2 antibody

N-CoR2 antibody

NCoR 2 antibody

Ncor2 antibody

NCOR2_HUMAN antibody

Nuclear Receptor Co-Repressor 2 antibody

Nuclear receptor corepressor 2 antibody

Expand

Images

  • Flow cytometric analysis of MG-63 cells labeling NCOR2/SMRT.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500226" style="font-weight: bold;text-decoration: underline;">HA500226</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor&reg; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of MG-63 cells labeling NCOR2/SMRT.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500226, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of NCOR2/SMRT on different lysates with Rabbit anti-NCOR2/SMRT antibody (<a href="/products/HA500226" style="font-weight: bold;text-decoration: underline;">HA500226</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /> <br />Exposure time: 60 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500226" style="font-weight: bold;text-decoration: underline;">HA500226</a>, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 273.7kDa<br />Observed band size: 237 kDa

    Western blot analysis of NCOR2/SMRT on different lysates with Rabbit anti-NCOR2/SMRT antibody (HA500226) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Exposure time: 60 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500226, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 273.7kDa
    Observed band size: 237 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"