NC2 alpha Rabbit Polyclonal Antibody
Catalog# HA500224
NC2 alpha Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NC2 alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human NC2 alpha aa 150-200.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 22 kDa
Positive Control
SW1990 cell lysate, HepG2 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.
Background References
1. Creton S. et. al. The NC2 alpha and beta subunits play different roles in vivo. Genes Dev. 2002 Dec
Subcellular Location
Nucleus.
Synonyms
DR1 associated corepressor antibody
DR1 associated protein 1 (negative cofactor 2 alpha) antibody
DR1 associated protein 1 antibody
DR1 associated protein1 antibody
Dr1-associated corepressor antibody
Dr1-associated protein 1 antibody
DRAP 1 antibody
Drap1 antibody
NC 2 alpha antibody
NC 2alpha antibody
ExpandImages
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Western blot analysis of NC2 alpha on different lysates with Rabbit anti-NC2 alpha antibody (HA500224) at 1/5,000 dilution.
Lane 1: SW1990 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 27 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500224) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"