SKI Rabbit Polyclonal Antibody
Catalog# HA500220
SKI Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SKI Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human SKI aa 250-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
80 kDa
Positive Control
HUVEC cell lysates, rat skeletal muscle tissue lysate, mouse kidney tissue lysate, human kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:100-1:500
Target
Function
This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation. May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Background References
1. Liao HY. et. al. Ski: Double roles in cancers. Clin Biochem. 2021 Jan
Subcellular Location
Nucleus.
Synonyms
Avian sarcoma viral (v ski) oncogene homolog antibody
C oncogene antibody
C ski antibody
Proto-oncogene c-Ski antibody
SKI antibody
Ski oncogene antibody
Ski oncoprotein antibody
SKI_HUMAN antibody
SKV antibody
v ski avian sarcoma viral oncogene homolog antibody
ExpandImages
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Western blot analysis of SKI on HUVEC cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500220, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of SKI on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500220, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat skeletal muscle tissue lysate
Lane 2: Mouse kidney tissue lysate -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SKI antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500220, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"